| Short Description : | Recombinant-Human PLAU/uPA (pro form)-C-His protein was developed in hek293 cells using the region Ser21-Leu431. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.22 Mu m filtered solution in 25 mM HEPES, 150 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | PLAU |
| Gene ID: | 5328 |
| Uniprot ID: | UROK_HUMAN |
| Immunogen: | Recombinant Human PLAU/uPA Protein (pro form) is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser21-Leu431) of Human PLAU/uPA (pro form) (Accession #P00749-1) fused with a C-His tag at the C-terminus. |
| Immunogen Region: | Ser21-Leu431 |
| Immunogen Sequence: | SNELHQVPSNCDCLNGGTCV SNKYFSNIHWCNCPKKFGGQ HCEIDKSKTCYEGNGHFYRG KASTDTMGRPCLPWNSATVL QQTYHAHRSDALQLGLGKHN YCRNPDNRRRPWCYVQVGLK LLVQECMVHDCADGKKPSSP PEELKFQCGQKTLRPRFKII GGEFTTIENQPWFAAIYRRH RGGSVTYVCGGSLISPCWVI SATHCFIDYPKKEDYIVYLG RSRLNSNTQGEMKFEVENL |
| Tissue Specificity | Expressed in the prostate gland and prostate cancers. |
| Post Translational Modifications | Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4. |
| Function | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. |
| Protein Name | Urokinase-Type Plasminogen ActivatorU-Plasminogen ActivatorUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-75205 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Urokinase-Type Plasminogen Activator proteinU-Plasminogen Activator proteinUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B proteinPLAU protein |
Information sourced from Uniprot.org
