Human PBEF/Visfatin/NAMPT protein (Recombinant) (N-Term 6×His tag) (STJP002916)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human PBEF/Visfatin/NAMPT Protein is produced by E. coli expression system. The target protein is expressed with sequence (Asn2-His491) of human Visfatin/Nampt (Accession #P43490) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Human PBEF/Visfatin/NAMPT-N-Term 6×His tag protein was developed in e.coli using the region Asn2-His491. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.2 Mu m filtered solution of 20mM HEPES, 150mM NaCl, pH 8.0. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured in a cell proliferation assay using RPMI 8226 cells. The ED50 for this effect is 1.72‑6.89 ng/mL, corresponding to a specific activity of 1.45×105~5.81×105units/mg. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | NAMPT |
| Gene ID | 10135 |
| Uniprot ID | NAMPT_HUMAN |
| Immunogen | Recombinant Human PBEF/Visfatin/NAMPT Protein is produced by E. coli expression system. The target protein is expressed with sequence (Asn2-His491) of human Visfatin/Nampt (Accession #P43490) fused with no additional amino acid. |
| Immunogen Region | Asn2-His491 |
| Immunogen Sequence | GGLRPNGQTKPLPALKLALE YIVPCMNKHGICVVDDFLGK ETGQQIGDEVRALHDTGKFT DGQLVSQKSDSSKDIRGDKI TWIEGKEPGCETIGLLMSSM DDLIRHCNGKLGSYKINGRT KAMVACYPGNGTGYVRHVDN PNGDGRCVTCIYYLNKDWDA KVSGGILRIFPEGKAQFADI EPKFDRLLFFWSDRRNPHEV QPAYATRYAITVWYFDADER ARAKVKYLTGEKGVRVELN |
Additional Info
| Tissue Specificity | Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. |
| Function | Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression. |
| Protein Name | Nicotinamide PhosphoribosyltransferaseNamprtaseNamptPre-B-Cell Colony-Enhancing Factor 1Pre-B Cell-Enhancing FactorVisfatin |
| Database Links | Reactome: R-HSA-1368108Reactome: R-HSA-197264Reactome: R-HSA-9768919 |
| Cellular Localisation | NucleusCytoplasmSecretedUnder Non-Inflammatory ConditionsVisfatin Predominantly Exhibits A Granular Pattern Within The NucleusSecreted By Endothelial Cells Upon Il-1beta StimulationAbundantly Secreted In MilkReaching 100-Fold Higher Concentrations Compared To Maternal Serum |
| Alternative Protein Names | Nicotinamide Phosphoribosyltransferase proteinNamprtase proteinNampt proteinPre-B-Cell Colony-Enhancing Factor 1 proteinPre-B Cell-Enhancing Factor proteinVisfatin proteinNAMPT proteinPBEF proteinPBEF1 protein |
Information sourced from Uniprot.org