Human Osteocalcin (Osteocalcin) Antibody Pair (STJAP00899)
General Information
| Short Description | This Osteocalcin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R03-8R-3/R09-5R-3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | BGLAP |
| Gene ID | 632 |
| Uniprot ID | OSTCN_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation by GGCX. These residues are essential for the binding of calcium . Decarboxylation promotes the hormone activity. |
| Function | Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation. Functions to limit bone formation without impairing bone resorption or mineralization. It binds strongly to apatite and calcium. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. |
| Protein Name | OsteocalcinBone Gla ProteinBgpGamma-Carboxyglutamic Acid-Containing Protein |
| Database Links | Reactome: R-HSA-159740Reactome: R-HSA-159763Reactome: R-HSA-159782Reactome: R-HSA-8940973 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Osteocalcin antibody pairBone Gla Protein antibody pairBgp antibody pairGamma-Carboxyglutamic Acid-Containing Protein antibody pairBGLAP antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Osteocalcin (Osteocalcin) Antibody Pair (STJAP00899)
This antibody pair detects human Osteocalcin (UniProt P02818) and is validated for human reactivity only. Reactivity in other species has not been confirmed for this product.
Both antibodies are unconjugated rabbit monoclonal IgGs, supplied in 0.01M PBS at pH 7.2, and purified by affinity chromatography. Clone IDs are R03-8R-3 and R09-5R-3 respectively.
This antibody pair is validated for use in ELISA. No other assay formats are specified for this product.
Optimal working concentrations are determined by the end user and are not pre-defined for this product. Titration experiments are recommended to establish the appropriate concentrations for each antibody in the intended assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to preserve activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.