Human NUDT16 protein (Recombinant) (His-Tag) (STJP017006)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human NUDT16-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 1mM DTT, 100mM NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NUDT16 |
| Gene ID | 131870 |
| Uniprot ID | NUD16_HUMAN |
| Accession Number | NP_689608 |
| Immunogen | Human |
| Immunogen Region | 1-195aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed strongly in lung, kidney, adrenal gland, testis, heart and brain. |
| Function | RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Also catalyzes the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA.metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Also acts as a phosphatase.hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. Exhibits decapping activity towards NAD-capped RNAs and FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro. |
| Protein Name | U8 Snorna-Decapping EnzymeIdp PhosphataseIdpaseInosine Diphosphate PhosphataseNucleoside Diphosphate-Linked Moiety X Motif 16Nudix Motif 16Nudix Hydrolase 16U8 Snorna-Binding Protein H29kM7gpppn-Mrna Hydrolase |
| Database Links | Reactome: R-HSA-2393930 |
| Cellular Localisation | NucleusNucleoplasmNucleolusCytoplasmLocalized Predominantly In The CytoplasmLocalized In NucleolusAnd In A Minor Proportion In Distinct Foci In The Nucleoplasm |
| Alternative Protein Names | U8 Snorna-Decapping Enzyme proteinIdp Phosphatase proteinIdpase proteinInosine Diphosphate Phosphatase proteinNucleoside Diphosphate-Linked Moiety X Motif 16 proteinNudix Motif 16 proteinNudix Hydrolase 16 proteinU8 Snorna-Binding Protein H29k proteinM7gpppn-Mrna Hydrolase proteinNUDT16 protein |
Information sourced from Uniprot.org