This NRG1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Human
Sensitivity
9.38pg/mL
Detection Limit
15.63~1000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells.inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction.stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells.stimulating Schwann cell proliferation.implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons.
Protein Name
Pro-Neuregulin-1 - Membrane-Bound IsoformPro-Nrg1 Cleaved Into - Neuregulin-1Acetylcholine Receptor-Inducing ActivityAriaBreast Cancer Cell Differentiation Factor P45Glial Growth FactorHeregulinHrgNeu Differentiation FactorSensory And Motor Neuron-Derived Factor
Pro-Neuregulin-1Membrane-Bound Isoform: Cell MembraneSingle-Pass Type I Membrane ProteinDoes Not Seem To Be ActiveNeuregulin-1: SecretedIsoform 8: NucleusMay Be NuclearIsoform 9: SecretedHas A Signal PeptideIsoform 10: MembraneMay Possess An Internal Uncleaved Signal Sequence
