| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Nectin-1 at 3 Mu g/mL (100 Mu L/well) can bind Recombinant Human Nectin-3 with a linear range of 180-720 ng/mL. |
| Gene Symbol: |
NECTIN3 |
| Gene ID: |
25945 |
| Uniprot ID: |
NECT3_HUMAN |
| Immunogen: |
Recombinant Human Nectin-3/PVRL3/CD113 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gly 58-Asp 400 ) of human Nectin 3 (Accession #NP_056295) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: |
GPIIVEPHVTAVWGKNVSLK CLIEVNETITQISWEKIHGK SSQTVAVHHPQYGFSVQGEY QGRVLFKNYSLNDATITLHN IGFSDSGKYICKAVTFPLGN AQSSTTVTVLVEPTVSLIKG PDSLIDGGNETVAAICIAAT GKPVAHIDWEGDLGEMESTT TSFPNETATIISQYKLFPTR FARGRRITCVVKHPALEKDI RYSFILDIQYAPEVSVTGYD GNWFVGRKGVNLKCNADAN |
| Tissue Specificity | Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines. |
| Function | Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types. Plays a role in the morphology of the ciliary body. |
| Protein Name | Nectin-3Cdw113Nectin Cell Adhesion Molecule 3Poliovirus Receptor-Related Protein 3Cd Antigen Cd113 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-420597 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinPostsynaptic Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionAdherens JunctionIn The Auditory EpitheliumSpecificaly Localizes To The Apical Side Of The Lateral Membranes Of Supporting Cells |
| Alternative Protein Names | Nectin-3 proteinCdw113 proteinNectin Cell Adhesion Molecule 3 proteinPoliovirus Receptor-Related Protein 3 proteinCd Antigen Cd113 proteinNECTIN3 proteinPRR3 proteinPVRL3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
