Human NAAA protein (Recombinant) (N-His) (STJP005144)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human NAAA-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | NAAA |
| Gene ID | 27163 |
| Uniprot ID | NAAA_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser29-Phe125 |
Additional Info
| Post Translational Modifications | N-glycosylated. Tunicamycin treatment causes a reduction in specific activity against N-palmitoylethanolamine. A disulfide bond is seen in the crystal structure of the human protein, but the Cys residues are not conserved in rodents. Autoproteolytic cleavage at pH 4.5 gives rise to the alpha and beta subunit. Cleavage gives rise to a conformation change that activates the enzyme. The same catalytic Cys residue mediates the autoproteolytic cleavage and subsequent hydrolysis of lipid substrates (Probable). |
| Function | Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine. |
| Protein Name | N-Acylethanolamine-Hydrolyzing Acid AmidaseAcid Ceramidase-Like ProteinAcylsphingosine Deacylase NaaaN-Acylsphingosine Amidohydrolase-LikeAsah-Like Protein Cleaved Into - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Alpha - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Beta |
| Database Links | Reactome: R-HSA-112310 |
| Cellular Localisation | LysosomeMembranePeripheral Membrane Protein |
| Alternative Protein Names | N-Acylethanolamine-Hydrolyzing Acid Amidase proteinAcid Ceramidase-Like Protein proteinAcylsphingosine Deacylase Naaa proteinN-Acylsphingosine Amidohydrolase-Like proteinAsah-Like Protein Cleaved Into - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Alpha - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Beta proteinNAAA proteinASAHL proteinPLT protein |
Information sourced from Uniprot.org