Human Mesothelin/MSLN protein (Recombinant) (C-hFc&Avi) {Biotin} (STJP020194)
SPECIFICATIONS
HostHEK293 cells
ConjugationBiotin
ImmunogenBiotinylated Recombinant Human Mesothelin/MSLN Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Glu296-Gly580) of Human Mesothelin fused with a hFc tag and Avi tag at the C-terminal.
General Information
| Short Description | Recombinant-Human Mesothelin/MSLN-C-hFc&Avi protein was developed in hek293 cells using the region Glu296-Gly580. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MSLN |
| Gene ID | 10232 |
| Uniprot ID | MSLN_HUMAN |
| Immunogen | Biotinylated Recombinant Human Mesothelin/MSLN Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Glu296-Gly580) of Human Mesothelin fused with a hFc tag and Avi tag at the C-terminal. |
| Immunogen Region | Glu296-Gly580 |
| Immunogen Sequence | EVEKTACPSGKKAREIDESL IFYKKWELEACVDAALLATQ MDRVNAIPFTYEQLDVLKHK LDELYPQGYPESVIQHLGYL FLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQVATLI DRFVKGRGQLDKDTLDTLTA FYPGYLCSLSPEELSSVPPS SIWAVRPQDLDTCDPRQLDV LYPKARLAFQNMNGSEYFVK IQSFLGGAPTEDLKALSQQN VSMDLATFMKLRTDAVLPL |
Additional Info
| Tissue Specificity | Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). |
| Post Translational Modifications | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. |
| Protein Name | MesothelinCak1 AntigenPre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating FactorMpf - Mesothelin - Cleaved Form |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorGolgi ApparatusMegakaryocyte-Potentiating Factor: SecretedIsoform 3: Secreted |
| Alternative Protein Names | Mesothelin proteinCak1 Antigen proteinPre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor proteinMpf - Mesothelin - Cleaved Form proteinMSLN proteinMPF protein |
Information sourced from Uniprot.org