Human MAGOH protein (Recombinant) (His-Tag) (STJP016678)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human MAGOH-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 0.1M NaCl, 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAGOH |
| Gene ID | 4116 |
| Uniprot ID | MGN_HUMAN |
| Accession Number | NP_002361 |
| Immunogen | Human |
| Immunogen Region | 1-146aa |
| Immunogen Sequence | MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S pre-initiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes).specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S).the function is different from the established EJC assembly. |
| Protein Name | Protein Mago Nashi Homolog |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9010553Reactome: R-HSA-975957 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmDetected In Granule-Like Structures In The DendroplasmTravels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To MrnaColocalizes With The Core EjcAlyref/Thoc4Nxf1 And Uap56 In The Nucleus And Nuclear Speckles |
| Alternative Protein Names | Protein Mago Nashi Homolog proteinMAGOH proteinMAGOHA protein |
Information sourced from Uniprot.org