Human LAG3 (Lymphocyte activation gene 3 protein) Sandwich ELISA Kit (STJE0020121)
SPECIFICATIONS
General Information
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 46.875 pg/mL |
| Detection Limit | 78.125-5000 pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | Store unopened box at 2-8 °C for up to 1 month. For longer storage check individual components. |
| Assay Time | 4 hours |
Target Information
| Gene Symbol | LAG3 |
| Gene ID | 3902 |
| Uniprot ID | LAG3_HUMAN |
| Specificity | This Sandwich ELISA kit recognizes Human Lymphocyte activation gene 3 protein in various sample types. No significant cross-reactivity or interference was observed between Human LAG3 analogues. |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
Additional Info
| Tissue Specificity | Primarily expressed in activated T-cells and a subset of natural killer (NK) cells. |
| Post Translational Modifications | Lymphocyte activation gene 3 protein: Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner. |
| Function | Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II).the precise role of MHC-II-binding is however unclear. Secreted lymphocyte activation gene 3 protein: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. |
| Protein Name | Lymphocyte Activation Gene 3 ProteinLag-3Cd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 ProteinSlag-3 |
| Database Links | Reactome: R-HSA-2132295 |
| Cellular Localisation | Lymphocyte Activation Gene 3 Protein: Cell MembraneSingle-Pass Type I Membrane ProteinSecreted Lymphocyte Activation Gene 3 Protein: SecretedProduced Following Cleavage Of The Main Chain |
| Alternative ELISA Names | Lymphocyte Activation Gene 3 Protein ELISA kitLag-3 ELISA kitCd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 Protein ELISA kitSlag-3 ELISA kitLAG3 ELISA kitFDC ELISA kit |
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Information sourced from Uniprot.org