| Short Description : | Recombinant-Human Interleukin-24-protein was developed from hek293. For use in research applications. |
| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation: | Lyophilised from 0.2 Mu m filtered solution in PBS, pH.4 including% (w/v) trehalose. |
| Storage Instruction: | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | Activity was determined by the ability to inhibit growth of human A549 cancer cells at concentrations ranging between-100 ng/ml. NA |
| Gene Symbol: | IL24 |
| Gene ID: | 11009 |
| Uniprot ID: | IL24_HUMAN |
| Immunogen: | Optimized DNA sequence encoding Human Interleukin-24 mature chain including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region: | Mature chain |
| Immunogen Sequence: | MNFQQRLQSL WTLARPFCPP LLATASQMQM VVLPCLGFTL LLWSQVSGAQ GQEFHFGPCQ VKGVVPQKLW EAFWAVKDTM QAQDNITSAR LLQQEVLQNV SDAESCYLVH TLLEFYLKTV FKNYHNRTVE VRTLKSFSTL ANNFVLIVSQ LQPSQENEMF SIRDSAHRRF LLFRRAFKQL DVEAALTKAL GEVDILLTWM QKFYKL NA |
| Tissue Specificity | Up-regulated in melanoma cells induced to terminally differentiate. |
| Post Translational Modifications | Glycosylated. Ubiquitination at Lys-122 promotes proteasomal degradation. |
| Function | Multifunctional cytokine mainly produced by T-cells that plays a regulatory role in immune response, tissue homeostasis, host defense, and oncogenesis. Possesses antiviral functions and induces the type I interferon response during influenza infection. Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1. In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1. Intracellularly, maintains endoplasmic reticulum homeostasis by restricting the eIF2alpha-CHOP pathway-mediated stress signal. In addition, acts as a quality control mechanism for the ubiquitin proteasome system by alerting the cell to proteasome dysfunction through activation of PKR/EIF2AK2. |
| Protein Name | Interleukin-24Il-24Melanoma Differentiation-Associated Gene 7 ProteinMda-7Suppression Of Tumorigenicity 16 Protein |
| Database Links | Reactome: R-HSA-8854691 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-24 proteinIl-24 proteinMelanoma Differentiation-Associated Gene 7 Protein proteinMda-7 proteinSuppression Of Tumorigenicity 16 Protein proteinIL24 proteinMDA7 proteinST16 protein |
Information sourced from Uniprot.org
