Human IL36A/IL-1F6 protein (Recombinant-Active) (Tag free) (STJP012766)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human IL36A/IL-1F6-Tag free protein was developed from e.coli and has a target region of Tag free. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in 20mM PB, 6% Trehalose, 5mM EDTA, 0.05% Tween 80, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8ยฐC for one week. Store at-20 to-80ยฐC for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/ยตg as determined by LAL test. |
Target Information
| Gene Symbol | IL36A |
| Gene ID | 27179 |
| Uniprot ID | IL36A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Lys6-Phe158 |
Additional Info
| Post Translational Modifications | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). |
| Function | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response.similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of pro-inflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes up-regulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by up-regulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in pro-inflammatory effects in the lung. |
| Protein Name | Interleukin-36 AlphaFil1 EpsilonInterleukin-1 EpsilonIl-1 EpsilonInterleukin-1 Family Member 6Il-1f6 |
| Database Links | Reactome: R-HSA-9014826 |
| Cellular Localisation | CytoplasmSecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Protein Names | Interleukin-36 Alpha proteinFil1 Epsilon proteinInterleukin-1 Epsilon proteinIl-1 Epsilon proteinInterleukin-1 Family Member 6 proteinIl-1f6 proteinIL36A proteinFIL1E proteinIL1E proteinIL1F6 protein |
Information sourced from Uniprot.org