| Short Description : | Recombinant-Human IL-5-C-His protein was developed in hek293 cells using the region Ile20-Ser134. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.5-2ng/mL. |
| Determination Method: | < 1.0 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | IL5 |
| Gene ID: | 3567 |
| Uniprot ID: | IL5_HUMAN |
| Immunogen: | Recombinant Human IL-5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile20-Ser134) of human IL-5 (Accession #NP_000870.1) fused with an 6×His tag at the C-terminus. |
| Immunogen Region: | Ile20-Ser134 |
| Immunogen Sequence: | IPTEIPTSALVKETLALLST HRTLLIANETLRIPVPVHKN HQLCTEEIFQGIGTLESQTV QGGTVERLFKNLSLIKKYID GQKKKCGEERRRVNQFLDYL QEFLGVMNTEWIIES |
| Tissue Specificity | Present in peripheral blood mononuclear cells. |
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5Il-5B-Cell Differentiation Factor IEosinophil Differentiation FactorT-Cell Replacing FactorTrf |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-5 proteinIl-5 proteinB-Cell Differentiation Factor I proteinEosinophil Differentiation Factor proteinT-Cell Replacing Factor proteinTrf proteinIL5 protein |
Information sourced from Uniprot.org
