| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human IL-4-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Immunoreactivity: |
Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 85-340 pg/mL, corresponding to a specific activity of 2.94 × 106~1.17× 107 units/mg. |
| Gene Symbol: |
IL4 |
| Gene ID: |
3565 |
| Uniprot ID: |
IL4_HUMAN |
| Immunogen Region: |
His25-Ser153 |
| Immunogen: |
Active Recombinant Human IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His25-Ser153) of human IL-4 (Accession #NP_000580.1) fused with a 6×His tag at the C-terminus. |
| Function | Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6. |
| Protein Name | Interleukin-4Il-4B-Cell Stimulatory Factor 1Bsf-1BinetrakinLymphocyte Stimulatory Factor 1Pitrakinra |
| Database Links | Reactome: R-HSA-6785807Reactome: R-HSA-9012546 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-4 proteinIl-4 proteinB-Cell Stimulatory Factor 1 proteinBsf-1 proteinBinetrakin proteinLymphocyte Stimulatory Factor 1 proteinPitrakinra proteinIL4 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
