Human IGFBP-6 protein (Active Recombinant) (C-His) (STJP000920)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IGFBP-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly240) of human IGFBP6/IBP-6 (Accession #NP_002169.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Active Recombinant-Human IGFBP-6-C-His protein was developed in hek293 cells using the region Met1-Gly240. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 Mu g/mL (100 Mu L/well) can bind recombinant human IGF1 with a linear range of 30-250 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IGFBP6 |
| Gene ID | 3489 |
| Uniprot ID | IBP6_HUMAN |
| Immunogen | Recombinant Human IGFBP-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly240) of human IGFBP6/IBP-6 (Accession #NP_002169.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Met1-Gly240 |
| Immunogen Sequence | MTPHRLLPPLLLLLALLLAA SPGGALARCPGCGQGVQAGC PGGCVEEEDGGSPAEGCAEA EGCLRREGQECGVYTPNCAP GLQCHPPKDDEAPLRALLLG RGRCLPARAPAVAEENPKES KPQAGTARPQDVNRRDQQRN PGTSTTPSQPNSAGVQDTEM GPCRRHLDSVLQQLQTEVYR GAQTLYVPNCDHRGFYRKRQ CRSSQGQRRGPCWCVDRMGK SLPGSPDGNGSSSCPTGSS |
Additional Info
| Post Translational Modifications | O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 6Ibp-6Igf-Binding Protein 6Igfbp-6 |
| Database Links | Reactome: R-HSA-381426 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Insulin-Like Growth Factor-Binding Protein 6 proteinIbp-6 proteinIgf-Binding Protein 6 proteinIgfbp-6 proteinIGFBP6 proteinIBP6 protein |
Information sourced from Uniprot.org