Human IFI35 protein (Recombinant) (N-His) (STJP005090)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human IFI35-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | IFI35 |
| Gene ID | 3430 |
| Uniprot ID | IN35_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser2-Gly286 |
Additional Info
| Post Translational Modifications | Phosphorylated. Dephosphorylation correlates with the formation of a complex with NMI. |
| Function | Acts as a signaling pathway regulator involved in innate immune system response. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response.the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation. In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production. In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion. Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines. |
| Protein Name | Interferon-Induced 35 Kda ProteinIfp 35Ifi-35 |
| Database Links | Reactome: R-HSA-909733 |
| Cellular Localisation | CytoplasmNucleusSecretedCytoplasmic Ifi35 Localizes In Punctate Granular StructuresNuclear Localization Increased Is Stimulated By Ifn-AlphaExtracelullar Following Secretion By Macrophage |
| Alternative Protein Names | Interferon-Induced 35 Kda Protein proteinIfp 35 proteinIfi-35 proteinIFI35 proteinIFP35 protein |
Information sourced from Uniprot.org