Human HINT1 protein (Recombinant) (No-Tag) (STJP016473)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human HINT1-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HINT1 |
| Gene ID | 3094 |
| Uniprot ID | HINT1_HUMAN |
| Accession Number | NP_005331 |
| Immunogen | Human |
| Immunogen Region | 1-126aa |
| Immunogen Sequence | MADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17. Deconjugates SUMO1 from RANGAP1. |
| Protein Name | Adenosine 5'-Monophosphoramidase Hint1Desumoylating Isopeptidase Hint1Histidine Triad Nucleotide-Binding Protein 1Protein Kinase C Inhibitor 1Protein Kinase C-Interacting Protein 1Pkci-1 |
| Database Links | Reactome: R-HSA-9824594Reactome: R-HSA-9825892Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmNucleusInteraction With Cdk7 Leads To A More Nuclear Localization |
| Alternative Protein Names | Adenosine 5'-Monophosphoramidase Hint1 proteinDesumoylating Isopeptidase Hint1 proteinHistidine Triad Nucleotide-Binding Protein 1 proteinProtein Kinase C Inhibitor 1 proteinProtein Kinase C-Interacting Protein 1 proteinPkci-1 proteinHINT1 proteinHINT proteinPKCI1 proteinPRKCNH1 protein |
Information sourced from Uniprot.org