| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human HAMP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
HAMP |
| Gene ID: |
57817 |
| Uniprot ID: |
HEPC_HUMAN |
| Specificity: |
This kit recognizes Human Hepc in samples. No significant cross-reactivity or interference between Human Hepc and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine and blood. Expressed by hepatocytes. |
| Function | Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa. |
| Protein Name | HepcidinLiver-Expressed Antimicrobial Peptide 1Leap-1Putative Liver Tumor RegressorPltr Cleaved Into - Hepcidin-25Hepc25 - Hepcidin-20Hepc20 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Hepcidin CLIA kitLiver-Expressed Antimicrobial Peptide 1 CLIA kitLeap-1 CLIA kitPutative Liver Tumor Regressor CLIA kitPltr Cleaved Into - Hepcidin-25 CLIA kitHepc25 - Hepcidin-20 CLIA kitHepc20 CLIA kitHAMP CLIA kitHEPC CLIA kitLEAP1 CLIA kitUNQ487 CLIA kitPRO1003 CLIA kit |
| Specificity | This kit recognizes Human Hepc in samples. No significant cross-reactivity or interference between Human Hepc and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-106 | 97 |
| EDTA plasma(n=8) | 97-111 | 104 |
| Cell culture media(n=8) | 99-114 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 92.27 | 211.26 | 844.52 | 89.92 | 190.73 | 828.61 |
| Standard deviation | 10.54 | 20.05 | 66.38 | 9.25 | 14.13 | 80.71 |
| CV (%) | 11.42 | 9.49 | 7.86 | 10.29 | 7.41 | 9.74 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
