Human GFR alpha-like/GFRAL protein (Recombinant) (C-His&Avi) (STJP020134)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human GFR alpha-like/GFRAL Protein is produced by mammalian expression system. The target protein is expressed with sequence (Ser19-Glu351) of Human GFR alpha-like/GFRAL (Accession #) fused with a His and Avi tag at the C-terminal. .
General Information
| Short Description | Recombinant-Human GFR alpha-like/GFRAL-C-His&Avi protein was developed in hek293 cells using the region Ser19-Glu351. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22um filtered solution in 20mM PB, 300mM NaCl (pH 6.5). Normally 5 % trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | GFRAL |
| Gene ID | 389400 |
| Uniprot ID | GFRAL_HUMAN |
| Immunogen | Recombinant Human GFR alpha-like/GFRAL Protein is produced by mammalian expression system. The target protein is expressed with sequence (Ser19-Glu351) of Human GFR alpha-like/GFRAL (Accession #) fused with a His and Avi tag at the C-terminal. . |
| Immunogen Region | Ser19-Glu351 |
Additional Info
| Tissue Specificity | Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius (at protein level). Detected at low levels in testis and adipose tissue. |
| Post Translational Modifications | Cleaved and inactivated by MMP14, inhibiting the GDF15-GFRAL aversive response. |
| Function | Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin. Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways. Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues. |
| Protein Name | Gdnf Family Receptor Alpha-Like |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinExtracellular Side |
| Alternative Protein Names | Gdnf Family Receptor Alpha-Like proteinGFRAL proteinC6orf144 proteinUNQ9356 proteinPRO34128 protein |
Information sourced from Uniprot.org