| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human GDF15-N-hFc protein was developed from hek293 and has a target region of N-hFc. For use in research applications. |
| Conjugation: |
Biotin |
| Formulation: |
Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: |
Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: |
The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: |
GDF15 |
| Gene ID: |
9518 |
| Uniprot ID: |
GDF15_HUMAN |
| Immunogen Region: |
Ala197-Ile308 |
| Immunogen: |
DNA sequence encoding Human GDF15 (Primary Amine Labeling) including a N-hFc tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Function | Hormone produced in response to various stresses to confer information about those stresses to the brain, and trigger an aversive response, characterized by nausea, vomiting, and/or loss of appetite. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. Acts by binding to its receptor, GFRAL, activating GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes responses to stressful conditions. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues. Required for avoidance behavior in response to food allergens: induced downstream of mast cell activation to promote aversion and minimize harmful effects of exposure to noxious substances. In addition to suppress appetite, also promotes weight loss by enhancing energy expenditure in muscle: acts by increasing calcium futile cycling in muscle. Contributes to the effect of metformin, an anti-diabetic drug, on appetite reduction and weight loss: produced in the kidney in response to metformin treatment, thereby activating the GDF15-GFRAL response, leading to reduced appetite and weight. The contribution of GDF15 to weight loss following metformin treatment is however limited and subject to discussion. Produced in response to anticancer drugs, such as camptothecin or cisplatin, promoting nausea, vomiting and contributing to malnutrition. Overproduced in many cancers, promoting anorexia in cancer (cachexia). Responsible for the risk of nausea and vomiting during pregnancy: high levels of GDF15 during pregnancy, mostly originating from the fetus, are associated with increased nausea and vomiting. Maternal sensitivity to nausea is probably determined by pre-pregnancy exposure to GDF15, women with naturally high level of GDF15 being less susceptible to nausea than women with low levels of GDF15 before pregnancy. Promotes metabolic adaptation in response to systemic inflammation caused by bacterial and viral infections in order to promote tissue tolerance and prevent tissue damage. Required for tissue tolerance in response to myocardial infarction by acting as an inhibitor of leukocyte integring activation, thereby protecting against cardiac rupture. Inhibits growth hormone signaling on hepatocytes. |
| Protein Name | Growth/Differentiation Factor 15Gdf-15Macrophage Inhibitory Cytokine 1Mic-1Nsaid-Activated Gene 1 ProteinNag-1Nsaid-Regulated Gene 1 ProteinNrg-1Placental Tgf-BetaPlacental Bone Morphogenetic ProteinProstate Differentiation Factor |
| Cellular Localisation | SecretedSecreted In The Plasma |
| Alternative Protein Names | Growth/Differentiation Factor 15 proteinGdf-15 proteinMacrophage Inhibitory Cytokine 1 proteinMic-1 proteinNsaid-Activated Gene 1 Protein proteinNag-1 proteinNsaid-Regulated Gene 1 Protein proteinNrg-1 proteinPlacental Tgf-Beta proteinPlacental Bone Morphogenetic Protein proteinProstate Differentiation Factor proteinGDF15 proteinMIC1 proteinPDF proteinPLAB proteinPTGFB protein |
Information sourced from Uniprot.org
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