Human FZD1 protein (Recombinant-Active) (C-hFc-His) (STJP012324)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human FZD1-C-hFc-His protein was developed from mammalian cells and has a target region of C-hFc-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFZD1 dilutions at 0.1-8, 000 ng/mL, the concentration of rhFrizzled-1/Fc Chimera that produces 50% of the optimal |
Target Information
| Gene Symbol | FZD1 |
| Gene ID | 8321 |
| Uniprot ID | FZD1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gln73-His253 |
Additional Info
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B. Contradictory results showing activation by WNT7B have been described for mouse. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. |
| Protein Name | Frizzled-1Fz-1Hfz1Fze1 |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-373080Reactome: R-HSA-4086400Reactome: R-HSA-4608870Reactome: R-HSA-4641262 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Protein Names | Frizzled-1 proteinFz-1 proteinHfz1 proteinFze1 proteinFZD1 protein |
Information sourced from Uniprot.org