Human FTL (Ferritin, Light Polypeptide) Sandwich ELISA Kit (STJE0019900)
SPECIFICATIONS
General Information
| Short Description | This FTL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.11 ng/mL |
| Detection Limit | 0.23-15 ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | FTL |
| Gene ID | 2512 |
| Uniprot ID | FRIL_HUMAN |
| Specificity | This kit recognizes Human FTL in samples. No significant cross-reactivity or interference between Human FTL and analogues was observed. |
| Sample Type | serum, plasma and other biological fluids |
Additional Info
| Function | Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron. |
| Protein Name | Ferritin Light ChainFerritin L Subunit |
| Database Links | Reactome: R-HSA-3000480Reactome: R-HSA-432722Reactome: R-HSA-6798695Reactome: R-HSA-917937 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosomeCytoplasmAutolysosome |
| Alternative ELISA Names | Ferritin Light Chain ELISA kitFerritin L Subunit ELISA kitFTL ELISA kit |
| Specificity | This kit recognizes Human FTL in samples. No significant cross-reactivity or interference between Human FTL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-99 | 92 |
| EDTA plasma (n=8) | 91-104 | 98 |
| Cell culture media (n=8) | 92-105 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.81 | 1.34 | 5.46 | 0.81 | 1.46 | 5.48 |
| Standard deviation | 0.05 | 0.06 | 0.16 | 0.05 | 0.07 | 0.26 |
| CV (%) | 5.68 | 4.13 | 3.02 | 6.33 | 4.49 | 4.72 |