| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 24.31pg/mL |
| Detection Limit: | 62.5-4000pg/mL |
| Short Description : | This FE Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum and plasma. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual. |
| Assay Time: | 1.5h |
| Detection: | Colormetric |
| Gene Symbol: | FTL FTH1 |
| Gene ID: | 2512 2495 |
| Uniprot ID: | FRIL_HUMAN FRIH_HUMAN |
| Specificity: | This kit recognizes Human FE in samples. No significant cross-reactivity or interference between Human FE and analogues was observed. |
| Sample Type: | Serum and plasma |
| Alternative ELISA Names | Ferritin Light Chain ELISA kit Ferritin L Subunit ELISA kit FTL ELISA kit Ferritin Heavy Chain ELISA kit Ferritin H Subunit ELISA kit Cell Proliferation-Inducing Gene 15 Protein Cleaved Into - Ferritin Heavy Chain - N-Terminally Processed ELISA kit FTH1 ELISA kit FTH ELISA kit FTHL6 ELISA kit OK ELISA kit SW-cl.84 ELISA kit PIG15 ELISA kit |
| Specificity | This kit recognizes FE in samples. No significant cross-reactivity or interference between FE and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T | 2-8℃, 1 months |
| Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials | 2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab (100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL | 2-8℃ (Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL | 2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL | |
| Concentrated Wash Buffer (25×) | 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL | |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL | 2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces96T*5: 25 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-101 | 92 |
| EDTA plasma(n=8) | 90-105 | 97 |
| Urine(n=8) | 91-105 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 183.00 | 362.63 | 1422.41 | 190.21 | 332.97 | 1312.84 |
| Standard deviation | 12.04 | 15.56 | 58.60 | 10.44 | 16.62 | 69.19 |
| CV (%) | 6.58 | 4.29 | 4.12 | 5.49 | 4.99 | 5.27 |