Human ELK1 protein (Recombinant) (N-His-SUMO) (STJP007989)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ELK1-N-His-SUMO protein was developed from e.coli for the region N-His-SUMO. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ELK1 |
| Gene ID | 2002 |
| Uniprot ID | ELK1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp2-Ser87 |
Additional Info
| Post Translational Modifications | Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1. Phosphorylated by PRP4K at Thr-417.phosphorylation activation ELK1 transcriptional activity. |
| Function | Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation. |
| Protein Name | Ets Domain-Containing Protein Elk-1 |
| Database Links | Reactome: R-HSA-198753Reactome: R-HSA-9031628Reactome: R-HSA-9609690Reactome: R-HSA-9634638 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Ets Domain-Containing Protein Elk-1 proteinELK1 protein |
Information sourced from Uniprot.org