| Short Description : | Recombinant-Human Dpolymerase delta subunit 4/POLD4-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications: | SDS-PAGE |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Concentration: | 0.25 mg/mL |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% Glycerol, 2mM DTT |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLD4 |
| Gene ID: | 57804 |
| Uniprot ID: | DPOD4_HUMAN |
| Accession Number: | NP_066996 |
| Immunogen: | Human |
| Immunogen Region: | 1-107aa |
| Immunogen Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked ubiquitination in response to UV irradiation, leading to proteasomal degradation. This modification is partly mediated by RNF8 and by the DCX(DTL) E3 ubiquitin ligase complex (also called CRL4(CDT2)). Efficient degradation requires the presence of PCNA and is required for the inhibition of fork progression after DNA damage. |
| Function | As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb. Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites. Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival. |
| Protein Name | Dna Polymerase Delta Subunit 4Dna Polymerase Delta Subunit P12 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-174411Reactome: R-HSA-174414Reactome: R-HSA-174417Reactome: R-HSA-174437Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5651801Reactome: R-HSA-5656169Reactome: R-HSA-5685942Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183 |
| Cellular Localisation | NucleusPartially Recruited To Dna Damage Sites Within 2 Hours Following Uv IrradiationBefore Degradation |
| Alternative Protein Names | Dna Polymerase Delta Subunit 4 proteinDna Polymerase Delta Subunit P12 proteinPOLD4 proteinPOLDS protein |
Information sourced from Uniprot.org
