Human DNA polymerase beta/POLB protein (Recombinant) (His-Tag) (STJP017317)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Dpolymerase beta/POLB-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% Glycerol, 0.1M NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLB |
| Gene ID | 5423 |
| Uniprot ID | DPOLB_HUMAN |
| Accession Number | NP_002681.1 |
| Immunogen | Human |
| Immunogen Region | 1-335aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation. |
| Function | Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase).through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase. |
| Protein Name | Dna Polymerase Beta5'-Deoxyribose-Phosphate Lyase5'-Drp LyaseAp Lyase |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-110373Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5651801Reactome: R-HSA-5689880Reactome: R-HSA-73930 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic In Normal ConditionsTranslocates To The Nucleus Following Dna Damage |
| Alternative Protein Names | Dna Polymerase Beta protein5'-Deoxyribose-Phosphate Lyase protein5'-Drp Lyase proteinAp Lyase proteinPOLB protein |
Information sourced from Uniprot.org