| Host: | Mammalian Cells |
| Applications: | ELISA/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Active-Human DKK4-C-10*His protein was developed from mammalian cells and has a target region of C-10*His. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit Wnt induced TCF reporter activity in human embryonic kidney cells (HEK293). Recombinant human DKK4 inhibits a constant dose of 500 ng/mL of recombinant human Wnt-3a. The ED50 for this effect is 150-900 ng/mL. |
| Gene Symbol: | DKK4 |
| Gene ID: | 27121 |
| Uniprot ID: | DKK4_HUMAN |
| Immunogen Region: | Leu19-Leu224 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Appears to be not glycosylated. Can be proteolytically processed by a furin-like protease. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
| Protein Name | Dickkopf-Related Protein 4Dickkopf-4Dkk-4Hdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3772470Reactome: R-HSA-5339717 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 4 proteinDickkopf-4 proteinDkk-4 proteinHdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form proteinDKK4 protein |
Information sourced from Uniprot.org