Human Dkk-3 protein (Recombinant) (C-His) (STJP000981)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Dkk-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala22-Ile350) of human Dkk-3/DKK3 (Accession #NP_037385.2 ) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Dkk-3-C-His protein was developed in hek293 cells using the region Ala22-Ile350. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | DKK3 |
| Gene ID | 27122 |
| Uniprot ID | DKK3_HUMAN |
| Immunogen | Recombinant Human Dkk-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala22-Ile350) of human Dkk-3/DKK3 (Accession #NP_037385.2 ) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Ala22-Ile350 |
| Immunogen Sequence | APAPTATSAPVKPGPALSYP QEEATLNEMFREVEELMEDT QHKLRSAVEEMEAEEAAAKA SSEVNLANLPPSYHNETNTD TKVGNNTIHVHREIHKITNN QTGQMVFSETVITSVGDEEG RRSHECIIDEDCGPSMYCQF ASFQYTCQPCRGQRMLCTRD SECCGDQLCVWGHCTKMATR GSNGTICDNQRDCQPGLCCA FQRGLLFPVCTPLPVEGELC HDPASRLLDLITWELEPDG |
Additional Info
| Tissue Specificity | Highest expression in heart, brain, and spinal cord. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
| Protein Name | Dickkopf-Related Protein 3Dickkopf-3Dkk-3Hdkk-3 |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 3 proteinDickkopf-3 proteinDkk-3 proteinHdkk-3 proteinDKK3 proteinREIC proteinUNQ258 proteinPRO295 protein |
Information sourced from Uniprot.org