Human DEFA5 protein (Recombinant) (N-His-SUMO) (STJP008859)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human DEFA5-N-His-SUMO protein was developed from e.coli for the region N-His-SUMO. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | DEFA5 |
| Gene ID | 1670 |
| Uniprot ID | DEF5_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Glu20-Arg94 |
Additional Info
| Post Translational Modifications | Glycosylated. Proteolytically cleaved at Arg-62 by trypsin. Both the propeptide form proHD5/HD5(20-94) and HD5(56-94) are cleaved into the lumenal peptide form HD5(63-94) by trypsin. Unprocessed proHD5 exerts antimicrobial activities, but peptide potency is enhanced by peptide processing. Proteolytically cleaved in duodenal fluid.derived fragments are antimicrobially active against commensal bacteria (in vitro). (Microbial infection) The disulfide bridges and homodimerization are a prerequisite for the enhancement of S.flexneri adhesion and invasion. |
| Function | Host-defense peptide that maintains sterility in the urogenital system. Has antimicrobial activity against a wide range of bacteria, including Gram-negative E.coli, P.aeruginosa and S.typhimurium, and Gram-positive E.aerogenes, S.aureus, B.cereus, E.faecium and L.monocytogenes. Confers resistance to intestinal infection by S.typhimurium. Exhibits antimicrobial activity against enteric commensal bacteria such as B.adolescentis, L.acidophilus, B.breve, L.fermentum, B.longum and S.thermophilus. Binds to bacterial membranes and causes membrane disintegration. Induces the secretion of the chemokine IL-8 by intestinal epithelial cells. Binds to B.antracis lef/lethal factor, a major virulence factor from B.anthracis, and neutralizes its enzymatic activity. (Microbial infection) Acts as a target for S.flexneri infection by binding to the bacterium, possibly via bacterial surface proteins, and thereby augmenting infectivity via enhanced bacterial adhesion and invasion of epithelial cells and tissues. |
| Protein Name | Defensin Alpha 5Defensin-5Hd5(20-94 Cleaved Into - Hd5(23-94 - Hd5(29-94 - Hd5(56-94 - Hd5(63-94 |
| Database Links | Reactome: R-HSA-1461973Reactome: R-HSA-1462054Reactome: R-HSA-9841251 |
| Cellular Localisation | SecretedCytoplasmic VesicleSecretory VesicleStored As Propeptide Hd5(20-94) In Secretory Granules Of Small Intestinal Paneth Cells And Found In The Ileum Lumen As Processed Mature PeptidesPredominantly In The Hd5(63-94) FormPeptides Hd5(20-94)Hd5(23-94) And Hd5(29-94) Are Found Within TissuesHd5(20-94) Being The Predominant Intracellular FormPeptides Hd5(56-94) And Hd5(63-94) Are Found In The Extracellular MilieuHd5(63-94) Being The Most Abundant FormSecreted Into The Female Genital Tract Lumen |
| Alternative Protein Names | Defensin Alpha 5 proteinDefensin-5 proteinHd5(20-94 Cleaved Into - Hd5(23-94 - Hd5(29-94 - Hd5(56-94 - Hd5(63-94 proteinDEFA5 proteinDEF5 protein |
Information sourced from Uniprot.org