| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Cystatin F-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate. The ED50 was measured to < 7nM NA |
| Gene Symbol: | CST7 |
| Gene ID: | 8530 |
| Uniprot ID: | CYTF_HUMAN |
| Immunogen Region: | ARG20-HIS145 |
| Immunogen: | Optimized DNA sequence encoding Human Cystatin F-CST7 (ARG20-HIS145 ) mature chain including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Primarily expressed in peripheral blood cells and spleen. |
| Function | Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system. |
| Protein Name | Cystatin-FCystatin-7Cystatin-Like Metastasis-Associated ProteinCmapLeukocystatin |
| Cellular Localisation | SecretedCytoplasm |
| Alternative Protein Names | Cystatin-F proteinCystatin-7 proteinCystatin-Like Metastasis-Associated Protein proteinCmap proteinLeukocystatin proteinCST7 protein |
Information sourced from Uniprot.org