| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is <11 nM. |
| Gene Symbol: | CST3 |
| Gene ID: | 1471 |
| Uniprot ID: | CYTC_HUMAN |
| Immunogen Region: | Met1-Ala146 |
| Immunogen: | Recombinant Human Cystatin-C/CST3 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Ala146) of human Cystatin C/CST3 (Accession #NP_000090.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | MAGPLRAPLLLLAILAVALA VSPAAGSSPGKPPRLVGGPM DASVEEEGVRRALDFAVGEY NKASNDMYHSRALQVVRARK QIVAGVNYFLDVELGRTTCT KTQPNLDNCPFHDQPHLKRK AFCSFQIYAVPWQGTMTLSK STCQDA |
| Post Translational Modifications | The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. |
| Function | As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. |
| Protein Name | Cystatin-CCystatin-3Gamma-TraceNeuroendocrine Basic PolypeptidePost-Gamma-Globulin |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-6798695Reactome: R-HSA-8957275Reactome: R-HSA-977225 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Cystatin-C proteinCystatin-3 proteinGamma-Trace proteinNeuroendocrine Basic Polypeptide proteinPost-Gamma-Globulin proteinCST3 protein |
Information sourced from Uniprot.org
