Human CXCL14 (C-X-C motif chemokine 14) CLIA Kit for Quantitative Detection

Name: Human CXCL14 (C-X-C motif chemokine 14) CLIA Kit for Quantitative Detection
SKU: STJC0000326
Availability: InStock

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SPECIFICATIONS

STJC0000326

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Short Description	This human CXCL14 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	CXCL14
Gene ID	9547
Uniprot ID	CXL14_HUMAN
Specificity	This kit recognizes Human BRAK/CXCL14 in samples. No significant cross-reactivity or interference between Human BRAK/CXCL14 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.
Post Translational Modifications	Ubiquitinated, followed by degradation by the proteasome.
Function	Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
Protein Name	C-X-C Motif Chemokine 14 Chemokine Brak Mip-2g Small-Inducible Cytokine B14
Database Links
Cellular Localisation	Secreted
Alternative CLIA Names	C-X-C Motif Chemokine 14 CLIA kit Chemokine Brak CLIA kit Mip-2g CLIA kit Small-Inducible Cytokine B14 CLIA kit CXCL14 CLIA kit MIP2G CLIA kit NJAC CLIA kit SCYB14 CLIA kit PSEC0212 CLIA kit UNQ240 CLIA kit PRO273 CLIA kit
Specificity	This kit recognizes Human BRAK/CXCL14 in samples. No significant cross-reactivity or interference between Human BRAK/CXCL14 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	103.36	176.33	819.52	106.88	177.41	873.36
Standard deviation	10.36	12.84	61.55	12.48	16.37	55.72
CV (%)	10.02	7.28	7.51	11.68	9.23	6.38