| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CXCL14 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL14 |
| Gene ID: |
9547 |
| Uniprot ID: |
CXL14_HUMAN |
| Specificity: |
This kit recognizes Human BRAK/CXCL14 in samples. No significant cross-reactivity or interference between Human BRAK/CXCL14 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. |
| Post Translational Modifications | Ubiquitinated, followed by degradation by the proteasome. |
| Function | Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. |
| Protein Name | C-X-C Motif Chemokine 14Chemokine BrakMip-2gSmall-Inducible Cytokine B14 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-X-C Motif Chemokine 14 CLIA kitChemokine Brak CLIA kitMip-2g CLIA kitSmall-Inducible Cytokine B14 CLIA kitCXCL14 CLIA kitMIP2G CLIA kitNJAC CLIA kitSCYB14 CLIA kitPSEC0212 CLIA kitUNQ240 CLIA kitPRO273 CLIA kit |
| Specificity | This kit recognizes Human BRAK/CXCL14 in samples. No significant cross-reactivity or interference between Human BRAK/CXCL14 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-106 | 100 |
| EDTA plasma(n=8) | 96-108 | 101 |
| Cell culture media(n=8) | 98-112 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.36 | 176.33 | 819.52 | 106.88 | 177.41 | 873.36 |
| Standard deviation | 10.36 | 12.84 | 61.55 | 12.48 | 16.37 | 55.72 |
| CV (%) | 10.02 | 7.28 | 7.51 | 11.68 | 9.23 | 6.38 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
