Human CTLA4 (CTLA4) Antibody Pair (STJAP00965)
General Information
| Short Description | This CTLA4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | CTLA4 |
| Gene ID | 1493 |
| Uniprot ID | CTLA4_HUMAN |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
| Database Links | Reactome: R-HSA-389356Reactome: R-HSA-389513Reactome: R-HSA-8877330 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
| Alternative Antibody Pair Names | Cytotoxic T-Lymphocyte Protein 4 antibody pairCytotoxic T-Lymphocyte-Associated Antigen 4 antibody pairCtla-4 antibody pairCd Antigen Cd152 antibody pairCTLA4 antibody pairCD152 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CTLA4 (CTLA4) Antibody Pair (STJAP00965)
This antibody pair is designed to detect human CTLA4 (UniProt P16410). Reactivity has been confirmed for human samples only.
The pair consists of two goat-derived antibodies: the capture antibody is unconjugated and supplied at 0.5 mg/mL, while the detection antibody is biotinylated and supplied at a concentration of 0.5 to 4 ug/mL.
This antibody pair has been validated for use in sandwich ELISA with colorimetric detection. No other assay formats are listed.
This antibody pair has been tested with serum, plasma, and other biological fluids. Researchers should validate performance in any additional sample types before use.
The recommended dilution range for use in sandwich ELISA is 1:1000 to 1:10000. Researchers may need to optimize dilutions depending on their specific experimental conditions.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.