Human CTLA4 (CD152) protein (Recombinant) (STJP000450)

Name: Human CTLA4 (CD152) protein (Recombinant) (STJP000450)
SKU: STJP000450
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

HostHEK293

ConjugationUnconjugated

ImmunogenDNA sequence encoding extracellular domain of Human CTLA4 to a C-terminal polyHis tag was expressed in HEK cells. NA

STJP000450

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Short Description	Recombinant-Human CTLA4 (CD152)-protein was developed from hek293. For use in research applications.
Host	HEK293
Note	STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Conjugation	Unconjugated
Dilution Range	Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Formulation	Lyophilised from 0.2 Mu m filtered PBS pH 7.4.
Storage Instruction	Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity	The activity was tested by immobilized recombinant CTLA4 (10ug/ml) ability to bind recombinant human B7-1 in functional ELISA. The ED50 was calculated to be 10ng/ml. NA

Gene Symbol	CTLA4
Gene ID	1493
Uniprot ID	CTLA4_HUMAN
Immunogen	DNA sequence encoding extracellular domain of Human CTLA4 to a C-terminal polyHis tag was expressed in HEK cells. NA
Immunogen Region	ECD

Tissue Specificity	Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
Post Translational Modifications	N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
Function	Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Protein Name	Cytotoxic T-Lymphocyte Protein 4 Cytotoxic T-Lymphocyte-Associated Antigen 4 Ctla-4 Cd Antigen Cd152
Database Links	Reactome: R-HSA-389356 Reactome: R-HSA-389513 Reactome: R-HSA-8877330
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Exists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization
Alternative Protein Names	Cytotoxic T-Lymphocyte Protein 4 protein Cytotoxic T-Lymphocyte-Associated Antigen 4 protein Ctla-4 protein Cd Antigen Cd152 protein CTLA4 protein CD152 protein

Information sourced from Uniprot.org