• Human COL6A3 (Collagen alpha-3 (VI) chain) CLIA Kit (STJC0000436)

Human COL6A3 (Collagen alpha-3 (VI) chain) CLIA Kit (STJC0000436)

SKU:
STJC0000436

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human COL6A3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: COL6A3
Gene ID: 1293
Uniprot ID: CO6A3_HUMAN
Specificity: This kit recognizes Human COL6 Alpha 3 in samples. No significant cross-reactivity or interference between Human COL6 Alpha 3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. The N-terminus is blocked.
Function Collagen VI acts as a cell-binding protein.
Protein Name Collagen Alpha-3(Vi Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Collagen Alpha-3(Vi Chain CLIA kit
COL6A3 CLIA kit
Specificity This kit recognizes Human COL6α3 in samples. No significant cross-reactivity or interference between Human COL6α3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-115 107
EDTA plasma(n=8) 87-100 92
Cell culture media(n=8) 91-103 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 45.73 150.54 490.98 44.06 163.90 468.93
Standard deviation 4.29 12.00 33.44 5.15 11.90 31.28
CV (%) 9.38 7.97 6.81 11.69 7.26 6.67
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance