| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CLDN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CLDN1 |
| Gene ID: |
9076 |
| Uniprot ID: |
CLD1_HUMAN |
| Specificity: |
This kit recognizes Human CLDN1 in samples. No significant cross-reactivity or interference between Human CLDN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. |
| Function | Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions. (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus. |
| Protein Name | Claudin-1Senescence-Associated Epithelial Membrane Protein |
| Database Links | Reactome: R-HSA-420029 |
| Cellular Localisation | Cell JunctionTight JunctionCell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneAssociates With Cd81 And The Cldn1-Cd81 Complex Localizes To The Basolateral Cell Membrane |
| Alternative CLIA Names | Claudin-1 CLIA kitSenescence-Associated Epithelial Membrane Protein CLIA kitCLDN1 CLIA kitCLD1 CLIA kitSEMP1 CLIA kitUNQ481 CLIA kitPRO944 CLIA kit |
| Specificity | This kit recognizes Human CLDN1 in samples. No significant cross-reactivity or interference between Human CLDN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-100 | 91 |
| EDTA plasma(n=8) | 97-108 | 103 |
| Cell culture media(n=8) | 96-111 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 100.24 | 311.52 | 795.72 | 92.50 | 327.00 | 852.76 |
| Standard deviation | 10.53 | 27.07 | 77.50 | 11.73 | 38.88 | 57.13 |
| CV (%) | 10.50 | 8.69 | 9.74 | 12.68 | 11.89 | 6.70 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
