Human CEA/CD66e protein (Recombinant) (C-His) (STJP001168)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CEA/CD66e Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys35-Ala685) of human CEACAM-5/CD66e (Accession #NP_004354.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CEA/CD66e-C-His protein was developed in hek293 cells using the region Lys35-Ala685. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CEACAM5 at 5 Mu g/mL (100 Mu L/well) can bind Recombinant Human CEACAM8 with a linear range of 0.5-3 Mu g/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CEACAM5 |
| Gene ID | 1048 |
| Uniprot ID | CEAM5_HUMAN |
| Immunogen | Recombinant Human CEA/CD66e Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys35-Ala685) of human CEACAM-5/CD66e (Accession #NP_004354.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Lys35-Ala685 |
| Immunogen Sequence | KLTIESTPFNVAEGKEVLLL VHNLPQHLFGYSWYKGERVD GNRQIIGYVIGTQQATPGPA YSGREIIYPNASLLIQNIIQ NDTGFYTLHVIKSDLVNEEA TGQFRVYPELPKPSISSNNS KPVEDKDAVAFTCEPETQDA TYLWWVNNQSLPVSPRLQLS NGNRTLTLFNVTRNDTASYK CETQNPVSARRSDSVILNVL YGPDAPTISPLNTSYRSGEN LNLSCHAASNPPAQYSWFV |
Additional Info
| Post Translational Modifications | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. |
| Function | Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression.induces resistance to anoikis of colorectal carcinoma cells. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. |
| Protein Name | Cell Adhesion Molecule Ceacam5Carcinoembryonic AntigenCeaCarcinoembryonic Antigen-Related Cell Adhesion Molecule 5Cea Cell Adhesion Molecule 5Meconium Antigen 100Cd Antigen Cd66e |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-202733 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorApical Cell MembraneCell SurfaceLocalized To The Apical Glycocalyx Surface |
| Alternative Protein Names | Cell Adhesion Molecule Ceacam5 proteinCarcinoembryonic Antigen proteinCea proteinCarcinoembryonic Antigen-Related Cell Adhesion Molecule 5 proteinCea Cell Adhesion Molecule 5 proteinMeconium Antigen 100 proteinCd Antigen Cd66e proteinCEACAM5 proteinCEA protein |
Information sourced from Uniprot.org