Human CDKN1B protein (Recombinant) (N-His) (STJP004766)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CDKN1B-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CDKN1B |
| Gene ID | 1027 |
| Uniprot ID | CDN1B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Thr198 |
Additional Info
| Post Translational Modifications | Phosphorylated.phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF. Ubiquitinated.in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated.by a TRIM21-containing SCF(SKP2)-like complex.leads to its degradation. Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation. |
| Function | Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1bCyclin-Dependent Kinase Inhibitor P27P27kip1 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-198323Reactome: R-HSA-2559582Reactome: R-HSA-2559586Reactome: R-HSA-5625900Reactome: R-HSA-5674400Reactome: R-HSA-6804116Reactome: R-HSA-69202Reactome: R-HSA-69231Reactome: R-HSA-69563Reactome: R-HSA-69656Reactome: R-HSA-8849470Reactome: R-HSA-9607240Reactome: R-HSA-9617828Reactome: R-HSA-9634638Reactome: R-HSA-9661069 |
| Cellular Localisation | NucleusCytoplasmEndosomeNuclear And Cytoplasmic In Quiescent CellsAkt- Or Rsk-Mediated Phosphorylation On Thr-198Binds 14-3-3Translocates To The Cytoplasm And Promotes Cell Cycle ProgressionMitogen-Activated Uhmk1 Phosphorylation On Ser-10 Also Results In Translocation To The Cytoplasm And Cell Cycle ProgressionPhosphorylation On Ser-10 Facilitates Nuclear ExportTranslocates To The Nucleus On Phosphorylation Of Tyr-88 And Tyr-89Colocalizes At The Endosome With Snx6This Leads To Lysosomal Degradation |
| Alternative Protein Names | Cyclin-Dependent Kinase Inhibitor 1b proteinCyclin-Dependent Kinase Inhibitor P27 proteinP27kip1 proteinCDKN1B proteinKIP1 proteinp27 protein |
Information sourced from Uniprot.org