Human CD74 protein (Recombinant) (His-Tag) (STJP018211)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human CD74-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Target Information
| Gene Symbol | CD74 |
| Gene ID | 972 |
| Uniprot ID | HG2A_HUMAN |
| Accession Number | NP_004346.1 |
| Immunogen | Human |
| Immunogen Region | 73-232aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Detected in urine (at protein level). Isoform p41: In B cells, represents 10% of total CD74 expression. Isoform p33: In B cells, represents 70% of total CD74 expression. |
| Post Translational Modifications | O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate. |
| Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Class-II-associated invariant chain peptide: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. Isoform p41: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform. |
| Protein Name | Hla Class Ii Histocompatibility Antigen Gamma ChainHla-Dr Antigens-Associated Invariant ChainIa Antigen-Associated Invariant ChainIiCd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain PeptideClip |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-2132295 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinEndoplasmic Reticulum MembraneGolgi ApparatusTrans-Golgi NetworkEndosomeLysosomeSecretedTransits Through A Number Of Intracellular Compartments In The Endocytic PathwayIt Can Either Undergo Proteolysis Or Reach The Cell MembraneIsoform P41: Late Endosome |
| Alternative Protein Names | Hla Class Ii Histocompatibility Antigen Gamma Chain proteinHla-Dr Antigens-Associated Invariant Chain proteinIa Antigen-Associated Invariant Chain proteinIi proteinCd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide proteinClip proteinCD74 proteinDHLAG protein |
Information sourced from Uniprot.org