Human CD177 protein (Recombinant) (C-hFc&His) (STJP001213)

General Information
Product Properties
Target Information
Additional Info
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Host:	HEK293 cells
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Recombinant-Human CD177-C-hFc&His protein was developed from hek293 cells and has a target region of C-hFc&His. For use in research applications.

Formulation:

Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.

Gene Symbol:	CD177
Gene ID:	57126
Uniprot ID:	CD177_HUMAN
Immunogen Region:	Met1-Gly407
Immunogen:	Recombinant Human CD177 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly407) of human CD177 (Accession #NP_065139.2.) fused with a Fc, 6×His tag at the C-terminus.

Tissue Specificity	Highly expressed in normal bone marrow and weakly expressed in fetal liver. During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level). Expressed by a subset of mature neutrophils (at protein level). The percentage of neutrophils expressing CD177 varies across the population. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
Post Translational Modifications	N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
Function	In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis.
Protein Name	Cd177 Antigen Human Neutrophil Alloantigen 2a Hna-2a Nb1 Glycoprotein Nb1 Gp Polycythemia Rubra Vera Protein 1 Prv-1 Cd Antigen Cd177
Database Links	Reactome: R-HSA-140875 Reactome: R-HSA-202733 Reactome: R-HSA-6798695
Cellular Localisation	Cell Membrane Lipid-Anchor Gpi-Anchor Membrane Raft Gpi-Like-Anchor Secreted Cytoplasmic Granule Membrane Cell Projection Lamellipodium Cell Surface Expression On Neutrophils Is Increased Upon Tnf-Alpha Fmlp Or Cxcl8/Il8-Mediated Stimulation In Neutrophils Stored Predominantly In Secondary And Tertiary Granules Can Also Be Shedded From The Cell Membrane Localizes To Lamellar Protrusions In Spreading Neutrophils
Alternative Protein Names	Cd177 Antigen protein Human Neutrophil Alloantigen 2a protein Hna-2a protein Nb1 Glycoprotein Nb1 Gp protein Polycythemia Rubra Vera Protein 1 protein Prv-1 protein Cd Antigen Cd177 protein CD177 protein NB1 protein PRV1 protein UNQ595 protein PRO1181 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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