| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human CD10 (Neprilysin)-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
0.2 Mu m filtered solution in 20mM Tris, 100mM NaCL, pH8.0 with 5% Trehalose. |
| Dilution Range: |
To prepare stock, reconstitute by gently mixing in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.DO NOT VORTEX |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with 0.1% HSA as carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
Measured by its ability to cleave flourogenic peptide substrate Mca-RPPGFSAFK (Dnp)-OH. The specific activity was measured to be >1nMole/min/ug NA |
| Gene Symbol: |
MME |
| Gene ID: |
4311 |
| Uniprot ID: |
NEP_HUMAN |
| Immunogen Region: |
ECD |
| Immunogen: |
Optimized DNA sequence encoding Human extracellular domain of human Neprilysin fused to a N-term polyHis tag was expressed in HEK293 cells. NA |
| Post Translational Modifications | Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. |
| Function | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. |
| Protein Name | NeprilysinAtriopeptidaseCommon Acute Lymphocytic Leukemia AntigenCallaEnkephalinaseNeutral Endopeptidase 24.11NepNeutral EndopeptidaseSkin Fibroblast ElastaseSfeCd Antigen Cd10 |
| Database Links | Reactome: R-HSA-2022377Reactome: R-HSA-5578768Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Protein Names | Neprilysin proteinAtriopeptidase proteinCommon Acute Lymphocytic Leukemia Antigen proteinCalla proteinEnkephalinase proteinNeutral Endopeptidase 24.11 proteinNep proteinNeutral Endopeptidase proteinSkin Fibroblast Elastase proteinSfe proteinCd Antigen Cd10 proteinMME proteinEPN protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
