| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This human CBLIF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CBLIF |
| Gene ID: |
2694 |
| Uniprot ID: |
IF_HUMAN |
| Specificity: |
This kit recognizes Human GIF in samples. No significant cross-reactivity or interference between Human GIF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Gastric mucosa. |
| Function | Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis. |
| Protein Name | Cobalamin Binding Intrinsic FactorGastric Intrinsic FactorIntrinsic FactorIfInf |
| Database Links | Reactome: R-HSA-3359457Reactome: R-HSA-3359462Reactome: R-HSA-3359463Reactome: R-HSA-9758881 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Cobalamin Binding Intrinsic Factor CLIA kitGastric Intrinsic Factor CLIA kitIntrinsic Factor CLIA kitIf CLIA kitInf CLIA kitCBLIF CLIA kitGIF CLIA kitIFMH CLIA kit |
| Specificity | This kit recognizes Human GIF in samples. No significant cross-reactivity or interference between Human GIF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-99 | 92 |
| EDTA plasma(n=8) | 95-112 | 102 |
| Cell culture media(n=8) | 100-114 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.83 | 6.37 | 16.25 | 1.93 | 6.42 | 16.73 |
| Standard deviation | 0.18 | 0.45 | 1.58 | 0.20 | 0.65 | 1.60 |
| CV (%) | 9.84 | 7.06 | 9.72 | 10.36 | 10.12 | 9.56 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
