Human CASP14 protein (Recombinant) (N-His) (STJP004551)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CASP14-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CASP14 |
| Gene ID | 23581 |
| Uniprot ID | CASPE_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Gln242 |
Additional Info
| Post Translational Modifications | Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts on the precursor to yield the p17/p10 mature form. Initially, cleavage between Ile-152 and Lys-153 has been proposed to yield the large and small subunits of the active enzyme. |
| Function | Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro has a preference for the substrate WY-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF. Involved in processing of prosaposin in the epidermis. May be involved in retinal pigment epithelium cell barrier function. Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD. |
| Protein Name | Caspase-14Casp-14 Cleaved Into - Caspase-14 Subunit P17 - Mature Form - Caspase-14 Subunit P10 - Mature Form - Caspase-14 Subunit P20 - Intermediate Form - Caspase-14 Subunit P8 - Intermediate Form |
| Database Links | Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Protein Names | Caspase-14 proteinCasp-14 Cleaved Into - Caspase-14 Subunit P17 - Mature Form - Caspase-14 Subunit P10 - Mature Form - Caspase-14 Subunit P20 - Intermediate Form - Caspase-14 Subunit P8 - Intermediate Form proteinCASP14 protein |
Information sourced from Uniprot.org