Human Carbonic anhydrase 2 protein (Active Recombinant) (No tag) (STJP000809)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human Carbonic anhydrase 2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser2-Lys260) of human Carbonic anhydrase II (Accession #NP_000058.1).
General Information
| Short Description | Active Recombinant-Human Carbonic anhydrase 2-No tag protein was developed in e.coli using the region Ser2-Lys260. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its esterase activity. The specific activity is >840 pmoles/min/Mu g, as measured with 1 mM 4-Nitrophenyl acetate and 0.1 Mu g enzyme at 400 nm in 100 Mu L of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CA2 |
| Gene ID | 760 |
| Uniprot ID | CAH2_HUMAN |
| Immunogen | Recombinant Human Carbonic anhydrase 2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser2-Lys260) of human Carbonic anhydrase II (Accession #NP_000058.1). |
| Immunogen Region | Ser2-Lys260 |
| Immunogen Sequence | SHHWGYGKHNGPEHWHKDFP IAKGERQSPVDIDTHTAKYD PSLKPLSVSYDQATSLRILN NGHAFNVEFDDSQDKAVLKG GPLDGTYRLIQFHFHWGSLD GQGSEHTVDKKKYAAELHLV HWNTKYGDFGKAVQQPDGLA VLGIFLKVGSAKPGLQKVVD VLDSIKTKGKSADFTNFDPR GLLPESLDYWTYPGSLTTPP LLECVTWIVLKEPISVSSEQ VLKFRKLNFNGEGEPEELM |
Additional Info
| Function | Catalyzes the reversible hydration of carbon dioxide. Can also hydrate cyanamide to urea. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Essential for bone resorption and osteoclast differentiation. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. |
| Protein Name | Carbonic Anhydrase 2Carbonate Dehydratase IiCarbonic Anhydrase CCacCarbonic Anhydrase IiCa-IiCyanamide Hydratase Ca2 |
| Database Links | Reactome: R-HSA-1237044Reactome: R-HSA-1247673Reactome: R-HSA-1475029 |
| Cellular Localisation | CytoplasmCell MembraneColocalized With Slc26a6 At The Surface Of The Cell Membrane In Order To Form A Bicarbonate Transport MetabolonDisplaced From The Cytosolic Surface Of The Cell Membrane By Pkc In Phorbol Myristate Acetate (Pma)-Induced Cells |
| Alternative Protein Names | Carbonic Anhydrase 2 proteinCarbonate Dehydratase Ii proteinCarbonic Anhydrase C proteinCac proteinCarbonic Anhydrase Ii proteinCa-Ii proteinCyanamide Hydratase Ca2 proteinCA2 protein |
Information sourced from Uniprot.org