| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human alpha 1 antichymotrypsin-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was measured by the ability of inhibit chymotrypsin cleavage of a fluorogenic peptide substrate, and the calulated ED50 was found to be < 6nM NA |
| Gene Symbol: | SERPINA3 |
| Gene ID: | 12 |
| Uniprot ID: | AACT_HUMAN |
| Immunogen Region: | HIS23-ALA423 |
| Immunogen: | Optimized DNA sequence encodingHuman alpha 1 antichymotrypsin-AACT (HIS23-ALA423) mature chain including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. |
| Protein Name | Alpha-1-AntichymotrypsinActCell Growth-Inhibiting Gene 24/25 ProteinSerpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Alpha-1-Antichymotrypsin proteinAct proteinCell Growth-Inhibiting Gene 24/25 Protein proteinSerpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less proteinSERPINA3 proteinAACT proteinGIG24 proteinGIG25 protein |
Information sourced from Uniprot.org