SDS-PAGE gel of recombinant human AKR1C3 protein 3μg Coomassie blue stain

Human Aldo-keto Reductase 1C3/AKR1C3 protein (Recombinant) (No-Tag) (STJP019260)

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STJP019260
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Short Description :Recombinant-Human Aldo-keto Reductase 1C3/AKR1C3-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications.
Applications:SDS-PAGE/Enzyme Activity
Host:E.coli
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Concentration:1 mg/mL
Formulation:Liquid in 20mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 10% Glycerol, 1mM DTT
Storage Instruction:For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin:< 1 EU per 1ug of protein (determined by LAL method)
Immunoreactivity:Specific activity is > 1, 000pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
Gene Symbol:AKR1C3
Gene ID:8644
Uniprot ID:AK1C3_HUMAN
Accession Number:NP_003730.4
Immunogen:Human
Immunogen Region:1-323aa
Immunogen Sequence:MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWV
Tissue Specificity Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. High expression in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
Function Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone. Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol. Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also displays retinaldehyde reductase activity toward 9-cis-retinal.
Protein Name Aldo-Keto Reductase Family 1 Member C3
17-Beta-Hydroxysteroid Dehydrogenase Type 5
17-Beta-Hsd 5
3-Alpha-Hsd Type Ii - Brain
3-Alpha-Hydroxysteroid Dehydrogenase Type 2
3-Alpha-Hsd Type 2
Chlordecone Reductase Homolog Hakrb
Dihydrodiol Dehydrogenase 3
Dd-3
Dd3
Dihydrodiol Dehydrogenase Type I
Ha1753
Prostaglandin F Synthase
Pgfs
Testosterone 17-Beta-Dehydrogenase 5
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-193807
Reactome: R-HSA-2162123
Reactome: R-HSA-5365859
Reactome: R-HSA-975634
Cellular Localisation Cytoplasm
Alternative Protein Names Aldo-Keto Reductase Family 1 Member C3 protein
17-Beta-Hydroxysteroid Dehydrogenase Type 5 protein
17-Beta-Hsd 5 protein
3-Alpha-Hsd Type Ii - Brain protein
3-Alpha-Hydroxysteroid Dehydrogenase Type 2 protein
3-Alpha-Hsd Type 2 protein
Chlordecone Reductase Homolog Hakrb protein
Dihydrodiol Dehydrogenase 3 protein
Dd-3 protein
Dd3 protein
Dihydrodiol Dehydrogenase Type I protein
Ha1753 protein
Prostaglandin F Synthase protein
Pgfs protein
Testosterone 17-Beta-Dehydrogenase 5 protein
AKR1C3 protein
DDH1 protein
HSD17B5 protein
KIAA0119 protein
PGFS protein

Information sourced from Uniprot.org

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