| Host: | E.coli |
| Applications: | SDS-PAGE/Enzyme Activity |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Aldo-keto Reductase 1B10/AKR1B10-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin: | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity: | Specific activity is > 1, 500pmol/min/ug, and was obtained by measuring the decrease of NADP in absorbance at 340nm resulting from NADPH at pH 7.0 at 37C. |
| Gene Symbol: | AKR1B10 |
| Gene ID: | 57016 |
| Uniprot ID: | AK1BA_HUMAN |
| Immunogen Region: | 1-316aa |
| Accession Number: | NP_064695 |
| Immunogen: | Human |
| Immunogen Sequence: | MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSL |
| Tissue Specificity | Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. |
| Function | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose. |
| Protein Name | Aldo-Keto Reductase Family 1 Member B10Arl-1Aldose Reductase-LikeAldose Reductase-Related ProteinArpHarpSmall Intestine ReductaseSi Reductase |
| Database Links | Reactome: R-HSA-975634 |
| Cellular Localisation | LysosomeSecretedSecreted Through A Lysosome-Mediated Non-Classical Pathway |
| Alternative Protein Names | Aldo-Keto Reductase Family 1 Member B10 proteinArl-1 proteinAldose Reductase-Like proteinAldose Reductase-Related Protein proteinArp proteinHarp proteinSmall Intestine Reductase proteinSi Reductase proteinAKR1B10 proteinAKR1B11 protein |
Information sourced from Uniprot.org