Human AIF1 (Allograft inflammatory factor 1) CLIA Kit for Quantitative Detection

Name: Human AIF1 (Allograft inflammatory factor 1) CLIA Kit for Quantitative Detection
SKU: STJC0000190
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000190

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

Short Description	This human AIF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	7.5pg/mL
Detection Limit	12.5~800pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	AIF1
Gene ID	199
Uniprot ID	AIF1_HUMAN
Specificity	This kit recognizes Human AIF1 in samples. No significant cross-reactivity or interference between Human AIF1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Detected in T-lymphocytes and peripheral blood mononuclear cells.
Post Translational Modifications	Phosphorylated on serine residues.
Function	Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Protein Name	Allograft Inflammatory Factor 1 Aif-1 Ionized Calcium-Binding Adapter Molecule 1 Protein G1
Database Links
Cellular Localisation	Cytoplasm Cytoskeleton Cell Projection Ruffle Membrane Peripheral Membrane Protein Cytoplasmic Side Phagocytic Cup Associated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis
Alternative CLIA Names	Allograft Inflammatory Factor 1 CLIA kit Aif-1 CLIA kit Ionized Calcium-Binding Adapter Molecule 1 CLIA kit Protein G1 CLIA kit AIF1 CLIA kit G1 CLIA kit IBA1 CLIA kit
Specificity	This kit recognizes Human AIF1 in samples. No significant cross-reactivity or interference between Human AIF1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	37.45	82.23	389.36	40.07	85.45	384.98
Standard deviation	4.36	6.46	40.42	3.94	7.58	38.84
CV (%)	11.64	7.86	10.38	9.83	8.87	10.09