Human AGR2 (Anterior gradient protein 2 homolog) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human AGR2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 18.75pg/mL |
| Detection Limit | 31.25~2000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | AGR2 |
| Gene ID | 10551 |
| Uniprot ID | AGR2_HUMAN |
| Specificity | This kit recognizes Human AGR2 in samples. No significant cross-reactivity or interference between Human AGR2 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. |
| Function | Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion. |
| Protein Name | Anterior Gradient Protein 2 HomologAg-2Hag-2Hpc8Secreted Cement Gland Protein Xag-2 Homolog |
| Database Links | |
| Cellular Localisation | SecretedEndoplasmic Reticulum |
| Alternative CLIA Names | Anterior Gradient Protein 2 Homolog CLIA kitAg-2 CLIA kitHag-2 CLIA kitHpc8 CLIA kitSecreted Cement Gland Protein Xag-2 Homolog CLIA kitAGR2 CLIA kitAG2 CLIA kitUNQ515 CLIA kitPRO1030 CLIA kit |
| Specificity | This kit recognizes Human AGR2 in samples. No significant cross-reactivity or interference between Human AGR2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-101 | 94 |
| EDTA plasma(n=8) | 84-98 | 91 |
| Cell culture media(n=8) | 92-109 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 110.65 | 170.57 | 861.73 | 114.70 | 186.33 | 864.08 |
| Standard deviation | 9.96 | 19.31 | 63.34 | 12.15 | 19.88 | 57.55 |
| CV (%) | 9.00 | 11.32 | 7.35 | 10.59 | 10.67 | 6.66 |