HRP-Conjugated Secondary Antibodies
Horseradish peroxidase conjugates for chromogenic and chemiluminescent detection
Horseradish peroxidase (HRP) is conjugated directly to the secondary antibody, where it acts on a chromogenic or chemiluminescent substrate to generate the signal used to visualise a target. The enzyme turns over substrate quickly, giving a strong signal in a short exposure, which makes HRP secondaries a standard choice for western blot, ELISA and immunohistochemistry. Our range covers HRP-conjugated secondary antibodies against mouse, rabbit, human and other host species, including chain-specific and HRP-polymer formats.
Browse HRP Secondary AntibodiesTwo Secondaries for the Most Common Detection Needs
A goat anti-mouse IgG (H&L) HRP conjugate is the standard secondary for detecting mouse monoclonal primaries by western blot, dot blot and ELISA. A goat anti-rabbit IgG (H) HRP-polymer conjugate carries multiple HRP molecules per antibody, which can increase the chemiluminescent signal obtained against low-abundance targets compared with a single-HRP conjugate.
Goat Anti-Mouse IgG H&L Antibody {HRP} (STJS000854)
A polyclonal HRP secondary raised in goat against mouse IgG heavy and light chains, validated by western blot against HeLa cell lysate. Used with a mouse-raised primary in western blot, dot blot or ELISA where a chemiluminescent or colourimetric readout is required.
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Goat Anti-Rabbit IgG H Heavy Chain Antibody {HRP Polymer} (STJS000859)
A goat anti-rabbit IgG heavy chain secondary built on an HRP-polymer backbone, validated by western blot across multiple lysates. Suited to rabbit-raised primaries where extra sensitivity is needed without switching to a longer exposure or a different primary dilution.
View product →Anti-Mouse HRP Secondary Antibodies
Anti-mouse HRP secondaries detect primary antibodies raised in mouse, the most common host species for monoclonal antibodies used in western blot, ELISA and immunohistochemistry. Our anti-mouse HRP conjugates are available against whole IgG (H+L), IgG2a, and against the isolated heavy or light chain, so a chain-specific secondary can be selected to avoid cross-reactivity with other antibodies present in the same blot or a co-immunoprecipitation workflow.
Whole IgG (H+L) conjugate, goat host.
Heavy chain-specific, avoids light chain cross-reactivity.
Light chain-specific, suits IP and co-IP blots.
Subclass-specific, for IgG2a primary antibodies.
Donkey host, alternative to goat-raised secondaries.
Anti-Rabbit, Anti-Human and Cross-Species HRP Secondary Antibodies
Anti-rabbit and anti-human HRP secondaries cover polyclonal primaries raised in rabbit and antibodies or immunoglobulins isolated from human serum. A donkey anti-rabbit IgG (H+L) conjugate detects rabbit primaries in western blot and paraffin-embedded IHC, while a goat anti-human IgG (H&L) conjugate detects human antibodies directly, for example when profiling patient serum or validating a human recombinant antibody. A chicken anti-goat IgG conjugate extends detection to goat-raised primaries, a host less often covered by standard rabbit and mouse secondary panels.
HRP-Conjugated Antibodies for Hepatitis B Research
Unlike the host-species secondaries above, these HRP conjugates bind directly to Hepatitis B viral antigens rather than to another antibody, giving a one-step readout in western blot, IHC, immunofluorescence and ELISA. HBsAg (surface antigen), HBeAg (e antigen) and HBcAg (core antigen) are the three markers most used to characterise HBV infection and replication in research samples, and each is available here as a direct HRP conjugate raised in goat.
Browse Other Secondary Antibody Conjugates
Where a fluorescent or particle-based readout suits the assay better than a chromogenic or chemiluminescent one, our secondary antibody range is also available in Alexa Fluor, Biotin, DyLight, gold-labelled and FITC formats. Select a conjugation type below to browse that part of the range.
Photostable fluorescent conjugates for imaging and flow cytometry.
Browse Alexa Fluor →Biotin conjugates for streptavidin and avidin-based detection systems.
Browse Biotinylated →Fluorescent conjugates across multiple wavelengths for multiplexed imaging.
Browse DyLight →Colloidal gold conjugates for electron microscopy and lateral flow formats.
Browse Gold-Labelled →Green-emitting fluorescent conjugates for IF and flow cytometry.
Browse FITC →Learning and Support
Choosing between an HRP and a fluorescent secondary conjugate?
An HRP secondary suits western blot, ELISA and chromogenic IHC, where a chemiluminescent or colourimetric substrate produces the readout. A fluorescent secondary such as DyLight, Alexa Fluor or FITC suits multiplexed immunofluorescence, where several targets are imaged at once in different channels.
Not sure which conjugation format fits your assay? Talk to us and our team can help you select the right product.
Looking for a Specific Reactivity or Host?
Browse the full range of HRP-conjugated secondary antibodies, or contact us if a specific reactivity, host species or chain specificity is not listed.
Browse HRP Secondary Antibodies